◔ Full-Time
➣ Location: Fenglin, Shanghai
☉ Junior Level
Responsibilities
- Design and execute autologous and in vivo CAR-T studies utilizing lentiviral vector (LVV) delivery systems.
- Carrying out lentiviral vector packaging and optimizing vector production, titer, and infectivity.
- Evaluate LVV transduction efficiency, tissue tropism, assess vector copy number (VCN), integration profile, and CAR expression kinetics/durability in primary cells and in vivo settings.
- Characterize CAR function through a suite of in vitro assays, including activation, cytotoxicity, and persistence profiles.
- Establish and qualify robust viral analytical methods for infectious/physical titers and stability testing.
- Perform safety assessments, including Replication Competent Lentivirus (RCL) testing and residuals/impurities analysis.
- Engineer cell lines using advanced gene editing technologies (e.g., CRISPR/Cas9) to support platform development.
- Analyze experimental data, generate high-quality figures, and prepare reports and presentations.
- Maintain accurate and complete experimental records in ELN, ensure compliance with BSL-2 practices, biosafety requirements and data integrity standards.
- Strong troubleshooting skills and ability to work independently.
Requirements
- Master’s degree or Ph.D. in a relevant discipline (Immunology, Cell/Molecular Biology, Biotechnology, or a related field).
- Hands-on experience with lentiviral vector (LVV) production, titration. Familiar with MOI optimization, transduction efficiency, vector copy number, RCL assessment.
- Experience with human primary immune cell isolation, culture, and phenotypic/functional characterization.
- Proficient in cell line development using gene-editing tools (e.g., CRISPR/Cas9, Lentiviral transduction) to create stable cell lines.
- Proficient in multicolor flow cytometry panel design, compensation, and gating strategies for immunophenotyping and CAR expression detection.
- Experienced in designing and executing potency assays, including cytotoxicity (luciferase/RTCA-based), cytokine profiling (ELISA/MSD/Luminex), T-cell persistence, and tumor rechallenge models.
- Skilled in PCR, qPCR, and ddPCR for evaluating Vector Copy Number (VCN) and in vivo biodistribution.
- Fluent in industry-standard software, including FlowJo, SnapGene, GraphPad Prism, and basic statistical packages. Experience with Electronic Lab Notebooks (ELN) and maintaining rigorous data integrity standards.
- Strong interpersonal skills with a demonstrated ability to work effectively in cross-functional teams.
- Proficient in English communication and writing.
If you are interested in joining our team, please send your resume to our HR department at nona.hr@nonabio.com.
